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Methods and Other Resources

Cryopreparation of cells for electron tomography. Yeast Protocols, CSHL Press, Submitted. O’Toole, E. T., Giddings, Jr., T. H., and Winey, M. (2014) Protocols for 3-D EM of yeast:

Building cell Structures in Three Dimensions: Electron tomography methods for budding yeast. Yeast Protocols, CSHL Press, Submitted. O’Toole, E. T., Giddings, Jr., T. H., and Winey, M. (2014)

Winey M, Meehl JB, O'Toole ET, Giddings TH Jr. Mol Biol Cell. 2014 Feb;25(3):319-23. doi: 10.1091/mbc.E12-12-0863. PMID:24482357

O'Toole ET, Giddings TH Jr, Porter ME, Ostrowski LE. Cytoskeleton (Hoboken). 2012 Aug;69(8):577-90. doi: 10.1002/cm.21035. Epub 2012 May 22. PMID:22573610

 McDonald K, Schwarz H, Müller-Reichert T, Webb R, Buser C, Morphew M. Methods Cell Biol. 2010;96:671-93. doi: 10.1016/S0091-679X(10)96028-7. PMID:20869543

 Giddings TH Jr, Meehl JB, Pearson CG, Winey M. Methods Cell Biol. 2010;96:117-41. doi: 10.1016/S0091-679X(10)96006-8. PMID: 20869521

 O'Toole ET. Methods Cell Biol. 2010;96:71-91. doi: 10.1016/S0091-679X(10)96004-4. PMID:20869519

 Meehl, J. B., Giddings, Jr., T. H., and Winey, M. (2009) Methods in Molecular Biology, 586:227-241. PMID: 19768433

 Morphew, M. He, W., Bjorkman, P.J., and McIntosh, J.R. (2008) J Microscopy, 230:263-267. PMID: 18445156

 O’Toole, E.T., Giddings, T.H. Jr., and Dutcher, S.K. (2007) Methods Cell Biol. 79, 125-143. PMID: 17327155

 McDonald KL, Morphew M, Verkade P, Müller-Reichert T. (2007). Methods Mol Biol. 369:143-73. PMID: 17656750

Morphew MK. (2007). Methods Cell Biol. 79:493-513. PMID: 17327171

 Giddings TH. J Microsc. 2003 Oct;212(Pt 1):53-61. PMID:14516362

 O'Toole, E. T., Winey, M., McIntosh, J. R., and Mastronarde, D. N. (2002) Methods Enzymol. 351:81-95.

 Giddings TH Jr, O'Toole ET, Morphew M, Mastronarde DN, McIntosh JR, Winey M. Methods Cell Biol. 2001;67:27-42. No abstract available. PMID:11550475

 O’Toole, E.T., D.N. Mastronarde, J.R. McIntosh, and M.E. Porter. 1995. Meth. Cell Biol. 47, 183-191. PMID;7476486

Sample Preparation for High Pressure Freezing

Practical Methods in High Pressure Freezing - Mary Morphew download