MACRO, Stereo, Brightfield (RGB color, epifluorescence)
FRANK - Zeiss Discovery.V12 fluorescent stereo (Porter B059)
Light Sources:
- Fostec Fiber Optic halogen illumination
Standard fluroescent filter configurations:
- GFP, AzamiGreen, Emerald, etc.
- mRFP, mCherry, TdTomato, etc.
• 1X Zeiss NA0.XX objective
• 8X - 100X via motorized optical zoom
• selectable display to indicate field size diameter, resolution
Nikon Digital Sight CCD camera (DS-Qi; 6.45 um x 6.45 um pixel)
- Fluorescent imaging of GFP and RFP and their related probes over a magnification range.
- Halogen illumination via Fostec Fiber Optic.
- NIS Elements BR 5.20.02 for fluorescence imaging (monochrome)
PEPPER - Nikon TiU widefield with RGB color brightfield (Porter B047A)
Light Sources:
- Halogen illumination for brightfield and DIC
Standard filter configurations:
Semrock BrightLine quad Sedat
- DAPI, Hoechst, etc.
- Alexa488, FITC, GFP, etc.
- TRITC, Alexa555, 568, 594, mRFP, mCherry, TdTomato, etc.
- Alexa647, Cy5, etc.
Standard objectives:
- 2x 0.10NA Plan Apo WD 8.5 (mm)
- 4x 0.13NA Plan Fluor WD 17.2 (mm)
- 10x 0.45NA Plan Apo DIC N1 WD 4 (mm)
- 20x 0.50NA Plan Fluor WD 2.1 (mm)
- 40x 0.60NA Plan Fluor WD 1.8 (mm)
- 100x 1.30NA Plan Fluor Ph3 OIL WD 0.20 (mm)
Optional objectives:
- 60x 1.40NA Plan Apo VC λ OIL WD 0.13 (mm)
- More (please inquire)
- for fluorescence
- for color brightfield (e.g. eosin, hematoxylin, crystal violet. etc.). This camera uses a CMOS chip with 5.9 megapixels.
motorized stage. Sample holders for standard slide, 35 and 60 mm dishes, and multiwell plates. DIC possible at 10x, and 60x. Brightfield color RGB imaging. Multi-point imaging. Montaging with stitching. Live cell imaging with TOKAI-HIT Cell Incubator; 1 X 35 (mm) dish or chamber slide (coverslip bottom). Rapid 4 color imaging via quad Sedat filter set (Semrock BrightLine).
MACRO - Olympus MVX10 MacroZoom fluorescent & color (Porter B059)
- Built in high-contrast white LED in base for transmission imaging
- CoolLED pE-300White: 400, 488, 561 nm LEDs
- ET-DAPI/FITC/TxRed triple Sedat filter set
Objectives (note, 2 position nose piece):
- Olympus 0.63X MVXPLAPO NA0.15 WD87mm (max field of view 55mm)
- Olympus 1X MVXPLAPO NA025 WD65mm (max field of view 34.9mm)
- Olympus 2X MVXPLAPO NA0.50 WD20mm (max field of view 17.6mm)
Zoom body:
- 0.63 - 6.3X
System magnifier:
- 2X
Effective magnification (to eyes; 10X less to camera when using 1X coupler and no system magnifier):
- Total system, 4 - 250X
- 0.63X objective, 4 - 80X
- 1X objective, 6.3 - 125X
- 2X objective 12.5 - 250X
Color RGB camera:
- , 1x1.8 inch 6.4MP (3088 x 2076 pixels) color CMOS, 2.4 micron x 2.4 micron pixels
Monochrome camera:
- 1`920 x 1200 pixels) CMOS, 5.86 x 5.86 micron pixels
System features:
- Sytem Macro and stereo viewing modes; macro maximizes brightness, stereo splits the light path to enhance depth of field
- Color camera is mounted with 0.63X adapter to maximize field size
- Monochrome camera is mounted at 1.0X distance, no glass to maximize brightness
- 2 position nose piece allows 2 objectives to be mounted simultaneouly
- Diaphragms to adjust brightness for both white light and fluorescece
- Triggered LED allow efficient blue, green, red imaging
- Customizable intensity for each LED to limit photobleaching
Capabilities:
- Base includes brightfield high-contrast, brightfield oblique, darkfield and blocking optics
- Time-lapse microscopy
- Multi-channel fluorescence
- Fiber optic white light (Fostec) for reflected light imaging
Olympus cellSens Standard version 4.2
